Unilab Clinical Laboratory

Test

Sample Requested

Special

Urine analysis

Urine, midstream urine, catheter, SPA.

Sterile container, proper wash of genitourinary section, no antibiotics.

Stool Examinations

Occult blood

Stool

No luxatives, meat, green vegetables, bananas, Fe tablets.

Ova, cysts, parasites

3 stool samples

Taken at different times.

Adeno-Rota

Stool

NONE

Culture

Stool

Preferably in sterile container.

Reducing Substances

Stool

NONE

FBC (and all individual components).

Wholeblood in EDTA

NONE

ESR

Wholeblood in EDTA or ESR bottle

NONE

Blood Groups

EDTA

NONE

Hb-electrophresis

EDTA

1 ml minimum

Sickle cell anemia

EDTA

NONE

Parasitic examination in blood

EDTA

NONE

PT/APTT

Citrated bottle (0.129 M)

Fill exactly stated volume

Clotting

Blood in capillary tube

NONE

Bleeding Time

Blood, filter paper

NONE

Fibrinogen

Citrated plasma

Fill exactly up to volume

HbA₁C

EDTA

NONE

Coombs indirect

(Rh antibodies)

Serum

NONE

Coombs direct

EDTA

NONE

NH₄

EDTA

Arterial blood – iced sample

Biochemistry

Preferably serum

(but plasma may be used)

Iron

Ferritin

Enzymes

    Only serum

Electrolytes

Calcium

Acid phosphatase

ALP NEVER IN EDTA

Electrolytes

Prolonged use of tourniquet contraindicated.

Calcium

Albumin

Calcium

Enzymes

Acid phosphatase

Avoid haemolysis

Lipids

Iron

Potassium

T. Protein

              CHOL /TRIG /HDL

      ------------------------------->

12-14 HR Fasting

Glucose

Fluoride Oxolate

(No anticoaqulant)------------>

If test not performed at once.

If to be tested at once.

Glucose Tolerance  test

Serum/Fluoride Oxalate

12 hour fasting.

Patient in the laboratory for length of the test.

B12

Serum

Freeze if not done immediately.

Folic Acid in RBC ’s

                 in  Serum

EDTA

Serum

NONE

G6PD        

EDTA

NONE

Allergy test

Serum

Min 5 ml

Protein Electrophoresis

Serum

NONE

Dpd

(Osteoporosis)

Urine

1^st morning sample

Serology

Serum

Immunology

Serum

NONE

Hormones

Serum

Cancer markers

Serum

NONE

Chlamydia I.F.

Swab: direct sample from urethra, cervix, rectum conjunctiva, nasopharynx.

Chlamydia

Serum

NONE

CSF

Culture/Biochemistry

CSF

Only by specialised physician

Calculus analysis

Renal calculi

NONE

Mycology

Hair, nails, skin scrapes

NONE

Wound swabs

Culture & Microscopy)

Sterile Swabs

Transport media

Sputum Culture

Sputum

Sterile container

TB (Microscopy &

Culture)

Urine, sputum, biopsy

Sterile container

24hr Creatinine / Calcium/ UA

24 hr urine

(in 2L bottles with HCL)

** Imlc. HCL 99ml H₂O or wash bottle with thymol

24 hr urine: Void first sample of day – collect for 24 hrs until the last collection of the 1^st urine of the second day.

VMA

24 hr urine

(in 2L bottles with HCL)

**

Samples as above.

(Avoid:coffee/tea, drugs, pineapple, avocado, coco, vanilla, cheese, nuts, chocolates, ice-cream, artificial flavourings, vinegar , carbonated drinks, candy, chewing gum. 

Albumin in urine

24 hr urine

(in 2L bottles with HCL)  **

Sample as above

Microalbumin in urine

Random urine

24 hr urine

(in 2L bottles with HCL)  **

Sample as above

HLA B27

ACD – 8 tube blood (5ml)

PCR

*   Ask   for   specific instructions by members of UNILAB

Water Chemical analysis

1½ Lt plastic bottle filled with water

Keep cool until transport to Lab / within 24 hrs.

Water Microbiological

5 x100 ml samples

Sterile container sampling by aseptic.

Technique: Use of alcohol  & burning of the tap. Allow wter to flow and collect after 1 min. in sterile containers using gloves. Transport in cooled system as soon as possible.

Food Samples

Food Stuff (at least 25 gms)

To be collected by member of UNILAB

Sterile containers and Forceps. Transportation in cooled system as soon as possible.

PTH(PARATHYROID HORMONE)

SERUM

After collection place in fridge for 30 mins ,then centrifuge and keep serum chilled until testing.